Wildlife Management Strategies Research Articles

Challenges and opportunities in human dimensions behind cat-wildlife conflict.

Because global anthropogenic activities cause vast biodiversity loss, human dimensions research is essential to forming management plans applicable to biodiversity conservation outside wilderness areas. Engaging public participation is crucial in this context to achieve social and environmental benefits. However, knowledge gaps remain in understanding how a balance between conservation and public demands can be reached and how complicated sociocultural contexts in the Anthropocene can be incorporated in conservation planning. We examined China's nationwide conflict between free-ranging cats (owned cats that are allowed to go outdoors or homeless cats living outdoors) and wildlife to examine how a consensus between compassion and biodiversity conservation can help in decision-making. We surveyed a random sample of people in China online. Over 9000 questionnaires were completed (44.2% response). In aggregate, respondents reported approximately 29 million free-ranging owned cats and that over 5 million domestic cats per year become feral in mainland China. Respondents who were cat owners, female, and religious were more likely to deny the negative impacts of cats on wildlife and ongoing management strategies and more supportive of stray cat shelters, adoption, and community-based fund raising than nonowners, male, and nonreligious respondents (p<0.05). Free-ranging cat ownership and abandonment occurred less with owners with more knowledge of biodiversity and invasive species than with respondents with less knowledge of these subjects (p<0.05). We recommend that cat enthusiasts and wildlife conservationists participate in community-based initiatives, such as campaigns to keep cats indoors. Our study provides a substantially useful framework for other regions where free-ranging cats are undergoing rapid expansion.

Human-geladas conflict and assessing local human attitudes for community-based conservation in Tigray region, Northern Ethiopia

ABSTRACT Understanding the nature and magnitude of human-wildlife conflict at the local level is critical for developing a wildlife management strategy. I used a questionnaire, focus group discussions, fecal dropping analyses, and direct observations to address the human-geladas conflict and promote the conservation of a relic monkey population in the Tigray region, Ethiopia. I analyzed data using descriptive statistics and chi-square tests. A high degree of human-geladas conflict was recorded as a result of habitat disturbance, agricultural expansion, and killing in response to crop-raiding. Geladas caused direct and perceived costs for the local people. The majority of the respondents from Hadnet (76%, n = 75) and Debre-Abay (71%, n = 74) villages had a negative attitude toward the geladas. To help reverse this sentiment and take steps toward reducing future human-geladas conflict, I recommend that local communities play a more active role in the management and conservation of the endemic geladas.

Assessing population structure and migration patterns of wild boar (Sus scrofa) in Japan

Geographical wildlife patterns reflect historical range expansion and connectivity and provide insights into wildlife population management. In our large-scale phylogeographic population analysis of wild boars (Sus scrofa leucomystax) in Japan, we identified 15 clusters using 29 microsatellite markers, each structured within a range of approximately 200 km. This suggests that evolution was essentially driven by isolation by distance, and that the range of gene flow was limited. One cluster contained subpopulations located approximately 900 km apart, indicating the occurrence of past anthropogenic introductions. Moreover, we estimated effective migration to visualize the geographic genetic population diversity. This analysis identified six potential barriers, one of which involved large plains and mountainous areas in the Kanto region of eastern Japan. This barrier likely persisted in the two eastern clusters for an extended period, restricting migration to the neighboring areas. Overall, our study sheds light on the demographic history of wild boar in Japan, provides evidence of past anthropogenic introductions from distant areas, and highlights the importance of geographic barriers in shaping genetic diversity and population dynamics. This knowledge will be beneficial for forming informed wildlife management strategies toward the conservation of genetic integrity and ecological balance of wild boar populations in Japan.

Hybrid mark–resight approaches account for heterogeneous detection bias in aerial surveys

Abstract Population abundance estimation is a critical yet challenging task for wildlife management decision‐making. For aerial surveys of terrestrial mammals in highly heterogenous landscapes, estimation models that address multiple sources of detection bias are needed. A typical hybrid mark–resight model includes an additive parameter that accounts for detection probabilities derived from marked animals. While this additive hybrid method improves abundance estimates, there remains a need to account for heterogeneity due to the conditional dependence in detection probability inherent in aerial surveys with multiple observers. We propose a modified approach, referred to as a conditional hybrid model, to address this heterogeneity directly. The conditional hybrid model applies a mark–resight approach typically used to account for tag loss in mark–recapture studies to aerial surveys with a subset of the population marked with GPS collars. We conduct a structured comparison between the additive and conditional hybrid estimation models and fit these two hybrid models to data from a 2020 aerial survey of mule deer in southern California. Both the additive and conditional hybrid models had general agreement in overall model structure and variable importance, however, the conditional model outperformed the additive model in capturing sources of detection heterogeneity and overall abundance estimates differed by 9%. Our top‐ranked models suggest that deer movement, larger group sizes, and rougher topography increased detection probability, whereas high cloud cover, obscuring vegetation and high observer fatigue and helicopter height decreased detection probability. Our findings illustrate the need to assess multiple correction methods when estimating abundance from aerial survey data to ensure accurate abundance estimates are being used to inform wildlife management strategies, with the caveat that specific survey conditions may dictate which method is the most appropriate for achieving robust estimates. These approaches represent the cutting‐edge science on wildlife abundance estimation from aerial survey data, a critical ingredient for effective wildlife management. By testing innovative hybrid methods, we present the first formal comparison of the strengths and weaknesses of each method within a highly heterogeneous landscape, providing practitioners with necessary information for future application.

Oxidative Status and Histological Evaluation of Wild Boars' Tissues Positive for Zearalenone Contamination in the Campania Region, Southern Italy.

Zearalenone (ZEN) is a mycotoxin produced by fungi belonging to the genera Fusarium spp. and commonly found in feed and food. It is frequently related to reproductive disorders in farm animals and, occasionally, to hyperestrogenic syndromes in humans. Nowadays, knowledge about ZEN effects on wild boars (Sus scrofa) is extremely scarce, despite the fact that they represent one of the most hunted game species in Italy. The aim of this study was to investigate how ZEN affects the liver, kidney, and muscle oxidative status and morphology of wild boars hunted in various locations throughout the province of Avellino, Campania Region, Southern Italy, during the 2021-2022 hunting season. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, as well as the malondialdehyde (MDA) levels, were assessed by colorimetric assays; tissue morphology was evaluated by hematoxylin-eosin and Masson's stains. Our data showed that ZEN contamination might result in oxidative stress (OS) and some histopathological alterations in wild boars' livers and kidneys rather than in muscles, emphasizing the importance of developing a wildlife monitoring and management strategy for dealing not only with the problem of ZEN but the surveillance of mycotoxins in general.

The Impact of Climate Change on Animal Health and Disease Patterns

Purpose: The aim of the study is to examine the impact of climate change on animal health and disease patterns&#x0D; Methodology: This study adopted a desktop methodology. This study used secondary data from which include review of existing literature from already published studies and reports that was easily accessed through online journals and libraries&#x0D; Findings: The study revealed that climate change has had a significant impact on animal health and disease patterns, with various negative consequences for biodiversity and ecosystems. Rising temperatures and changing rainfall patterns influence the distribution and abundance of disease vectors, such as mosquitoes and ticks and alter their geographic ranges. This can result in the spread of diseases like malaria, dengue fever, Lyme disease, and West Nile virus to new areas. Additionally, climate change enhance the replication and survival rates of pathogens, potentially leading to more frequent and severe disease outbreaks.&#x0D; Unique Contribution to Theory, Practice and Policy: The study was anchored on One Health theory and Stressor-response theory. The study recommends Incorporation of climate change considerations into wildlife conservation and management strategies. This involves protecting and restoring habitats, creating wildlife corridors to facilitate species movement, and implementing adaptive management practices that consider the potential impacts of climate change on disease dynamics. Conservation efforts should prioritize the preservation of genetic diversity and the enhancement of resilience in animal populations.

Conservation management strategy impacts inbreeding and mutation load in scimitar-horned oryx

In an age of habitat loss and overexploitation, small populations, both captive and wild, are increasingly facing the effects of isolation and inbreeding. Genetic management has therefore become a vital tool for ensuring population viability. However, little is known about how the type and intensity of intervention shape the genomic landscape of inbreeding and mutation load. We address this using whole-genome sequence data of the scimitar-horned oryx (Oryx dammah), an iconic antelope that has been subject to contrasting management strategies since it was declared extinct in the wild. We show that unmanaged populations are enriched for long runs of homozygosity (ROH) and have significantly higher inbreeding coefficients than managed populations. Additionally, despite the total number of deleterious alleles being similar across management strategies, the burden of homozygous deleterious genotypes was consistently higher in unmanaged groups. These findings emphasize the risks associated with deleterious mutations through multiple generations of inbreeding. As wildlife management strategies continue to diversify, our study reinforces the importance of maintaining genome-wide variation in vulnerable populations and has direct implications for one of the largest reintroduction attempts in the world.

Disgust in animals and the application of disease avoidance to wildlife management and conservation.

Disgust is an adaptive system hypothesized to have evolved to reduce the risk of becoming sick. It is associated with behavioural, cognitive and physiological responses tuned to allow animals to avoid and/or get rid of parasites, pathogens and toxins. Little is known about the mechanisms and outcomes of disease avoidance in wild animals. Furthermore, given the escalation of negative human-wildlife interactions, the translation of such knowledge into the design of evolutionarily relevant conservation and wildlife management strategies is becoming urgent. Contemporary methods in animal ecology and related fields, using direct (sensory cues) or indirect (remote sensing technologies and machine learning) means, provide a flexible toolbox for testing and applying disgust at individual and collective levels. In this review/perspective paper, we provide an empirical framework for testing the adaptive function of disgust and its associated disease avoidance behaviours across species, from the least to the most social, in different habitats. We predict various trade-offs to be at play depending on the social system and ecology of the species. We propose five contexts in which disgust-related avoidance behaviours could be applied, including endangered species rehabilitation, invasive species, crop-raiding, urban pests and animal tourism. We highlight some of the perspectives and current challenges of testing disgust in the wild. In particular, we recommend future studies to consider together disease, predation and competition risks. We discuss the ethics associated with disgust experiments in the above contexts. Finally, we promote the creation of a database gathering disease avoidance evidence in animals and its applications.

Nutritional properties of spectacled caiman (Caiman yacare) meat marketed as part of a wildlife management strategy in the Beni river basin

We determined the nutritional quality of yacare meat (Caiman yacare) in the Beni river basin. Commercial cut samples were obtained from the tails of four males harvested as part of a management plan implemented in the Tacana Indigenous Territory. This plan guarantees long-term sustainability of caiman hunting. pH analysis, the Eber test, and the ammoniacal nitrogen test suggest that the meat was of good quality, processed properly, and free of contamination. Nutritional analyses suggest that yacare meat is a healthy alternative for human consumption, as it has a high protein value (23.02 g/100g) and low fat concentration (0.67g/100g). Yacare meat has a high concentration of minerals such as phosphorus (227.53 g) and calcium (11.84 g), both beneficial for memory and cardiovascular health. Caiman meat can be considered as a valuable nutritional supplement in human diets both in urban and rural areas

Severe environmental conditions create severe conflicts: A novel ecological pathway to extreme coyote attacks on humans

Abstract Identifying the circumstances and causes of carnivore attacks on humans is important for prevention of future incidents as well as employing effective wildlife management strategies. Cape Breton Highlands National Park (CBHNP) in Nova Scotia has experienced multiple attacks by coyotes Canis latrans on humans, including a fatal attack on an adult in 2009. Here we use a combination of data on space use and diet collected from 2011 to 2013 to reveal that limited resources and a reliance on a large ungulate (moose, Alces americanus) as the mechanism leading to aggression by coyotes in CBHNP. Resident coyotes exhibited large home range sizes (mean = 77.5 km2) indicative of limited resources and spatiotemporal avoidance of human activity. Carbon (δ13C) and nitrogen (δ15N) isotope values of sub‐sampled coyote whiskers (n = 32), which provide a longitudinal record of diet over the months before collection, revealed little intra‐ and inter‐individual variation with nearly all individuals specializing on moose, a pattern that agrees with indices of natural resource availability. Specifically, stable isotope mixing models show that moose was the most important prey for most coyotes (25/32), representing between 41% and 78% of dietary inputs. Only four coyotes exhibited use of anthropogenic resources (human foods), and only one of seven coyotes involved in attacks on people had been consuming human foods before the attacks. Synthesis and Applications: We have described a unique ecological system in which a generalist carnivore has expanded its niche to specialize on a large prey species, with the unfortunate consequence of also expanding pathways to conflicts with people. Our results suggest extreme unprovoked predatory attacks by coyotes on people are likely to be quite rare and associated with unique ecological characteristics. Extreme management actions such as bounties are unnecessary, but managers may need to employ hazing or lethal removal earlier in the conflict process than under normal circumstances. Also, users of these areas should be made aware of the risks coyotes pose and encouraged to take precautions.

Red Deer in Lithuania: History, Status and Management

The history, current population status and (un)sustainable management of red deer (Cervus elaphus) in Lithuania are reviewed on the basis of 57 publications and, additionally, analysis of data on numbers and hunting bag. After the extinction of the species at the beginning of the 19th century, red deer were reintroduced into the northern part of the country during World War I. Population re-establishment was further fueled after WWII by immigration from neighboring countries and local translocations. After the introduction of the Law on Hunting in 2002, which enabled hunting organizations to rent hunting grounds for longer than 10 years, local wildlife management strategies were targeted at increasing the number of animals. However, a scientifically-based target population size for C. elaphus, agreed by all major stakeholders, has still not been defined. In the last 20 years, population growth has been exponential, deer numbers in Lithuania being estimated as 68,816 individuals in 2021. An increase in the size of the hunting bag is necessary for the sustainable management of the species at the national scale and to avoid risks of over-population as observed elsewhere in Europe.

Integrating terrestrial scavenging ecology into contemporary wildlife conservation and management.

Scavenging plays a vital role in maintaining ecosystem health and contributing to ecological functions; however, research in this sub‐discipline of ecology is underutilized in developing and implementing wildlife conservation and management strategies. We provide an examination of the literature and recommend priorities for research where improved understanding of scavenging dynamics can facilitate the development and refinement of applied wildlife conservation and management strategies. Due to the application of scavenging research broadly within ecology, scavenging studies should be implemented for informing management decisions. In particular, a more direct link should be established between scavenging dynamics and applied management programs related to informing pharmaceutical delivery and population control through bait uptake for scavenging species, prevention of unintentional poisoning of nontarget scavenging species, the epidemiological role that scavenging species play in disease dynamics, estimating wildlife mortalities, nutrient transfer facilitated by scavenging activity, and conservation of imperiled facultative scavenging species. This commentary is intended to provide information on the paucity of data in scavenging research and present recommendations for further studies that can inform decisions in wildlife conservation and management. Additionally, we provide a framework for decision‐making when determining how to apply scavenging ecology research for management practices and policies. Due to the implications that scavenging species have on ecosystem health, and their overall global decline as a result of anthropic activities, it is imperative to advance studies in the field of scavenging ecology that can inform applied conservation and management programs.

Chromosomal-level reference genome assembly of the North American wolverine (Gulo gulo luscus): a resource for conservation genomics.

We report a chromosomal-level genome assembly of a male North American wolverine (Gulo gulo luscus) from the Kugluktuk region of Nunavut, Canada. The genome was assembled directly from long-reads, comprising: 758 contigs with a contig N50 of 36.6 Mb; contig L50 of 20; base count of 2.39 Gb; and a near complete representation (99.98%) of the BUSCO 5.2.2 set of 9,226 genes. A presumptive chromosomal-level assembly was generated by scaffolding against two chromosomal-level Mustelidae reference genomes, the ermine and the Eurasian river otter, to derive a final scaffold N50 of 144.0 Mb and a scaffold L50 of 7. We annotated a comprehensive set of genes that have been associated with models of aggressive behavior, a trait which the wolverine is purported to have in the popular literature. To support an integrated, genomics-based wildlife management strategy at a time of environmental disruption from climate change, we annotated the principal genes of the innate immune system to provide a resource to study the wolverine’s susceptibility to new infectious and parasitic diseases. As a resource, we annotated genes involved in the modality of infection by the coronaviruses, an important class of viral pathogens of growing concern as shown by the recent spillover infections by severe acute respiratory syndrome coronavirus-2 to naïve wildlife. Tabulation of heterozygous single nucleotide variants in our specimen revealed a heterozygosity level of 0.065%, indicating a relatively diverse genetic pool that would serve as a baseline for the genomics-based conservation of the wolverine, a rare cold-adapted carnivore now under threat.

A demographic model to support customary management of a culturally important waterfowl species

Scientific support invited by Indigenous peoples and local communities (IPLC) to assist with customary environmental management can improve conservation and community livelihoods. For example, demographic models can help to understand how alternative wildlife management strategies affect population dynamics and harvest sustainability. We developed a demographic model to assist Ngāi Tahu, the southern-most Māori tribe in Aotearoa/New Zealand, in customary management of a culturally important population of Black Swans (<em>kakī anau</em>, <em>Cygnus atratus</em>). We used recent demographic data, including results of an experimental egg harvest study, to inform <em>tangata tiaki</em> (Ngāi Tahu environmental guardians) about how customary egg harvest and background pressure from sport hunting of swans aged ≥ 1 year differentially affect population growth. We also assessed how sport hunting of swans affects the sustainability of customary egg harvest. Estimated population growth (1.018 or presently growing 1.8% annually; 95% CI: 0.808–1.241) was most sensitive to changes in adult and subadult survival, followed by juvenile (first-year) survival, breeding propensity, and nest hatching success. Uncertainty in population growth was almost entirely attributable to uncertainty in swan survival rates after hatching. Sustainable population-level rates of egg harvest varied from none to more than half of all eggs, depending on small changes in adult and subadult survival. Population sensitivity to adult and subadult survival suggests that limiting and monitoring their mortality are crucial to population and egg harvest sustainability, whereas contemporary government-mandated species management, through Fish and Game New Zealand, allows adult and subadult mortality from sport hunting, with little record of offtake. Recognizing the rights and interests of Ngāi Tahu, and monitoring swan mortality more closely, could improve Ngāi Tahu abilities to practice customary harvest, enhance population and environmental monitoring, and, when appropriate, control swan numbers in a culturally appropriate and less wasteful way. The model we present could aid decision making and communication between Ngāi Tahu and New Zealand’s Crown government within a potential future co-management arrangement. Demographic models can be useful tools for supporting customary environmental management, but developing, maintaining, and implementing these tools requires support for adaptive policies and management arrangements that recognize IPLC rights to the environment and decision making.

Habitat connectivity and resource selection in an expanding bobcat (Lynx rufus) population.

Terrestrial carnivores are among the most imperiled species worldwide, yet some species are resilient and are recovering in human-dominated landscapes after decades or centuries of absence. Bobcat (Lynx rufus) populations were extirpated from much of Midwestern US in the mid-1800’s, and are currently expanding and recolonizing their former range. In this study, we investigated multi-scale habitat selection for Ohio’s expanding bobcat population, and examined habitat connectivity in order to evaluate the conduits for dispersal statewide. We used citizen observations collected between 1978 and 2019 and logistic regression to evaluate population-level habitat selection, and GPS telemetry data for 20 individuals collected between 2012 and 2014 and a distribution-weighted exponential Resource Selection Function to evaluate individual-level habitat selection within home ranges. At the population level, bobcats selected for higher amounts of forest and pasture (at a 50 km2 scale) and herbaceous vegetation (at 15–50 50 km2 scales), thus overall heterogeneous forested habitat. At individual (home range) level, bobcats selected for forested habitats with low road density and farther away from high traffic roads; they also showed weak selection for open habitat at the home range level. Male home ranges were significantly greater than female home ranges. Lastly, we used the population-level spatial outputs (i.e. habitat suitability map) to parameterize habitat connectivity models using circuit theory in the program Circuitscape. We tested three relationships between habitat suitability and resistance to movement and used a subset of data on potential dispersing individuals to evaluate which relationship performed best. All three relationships performed almost equally well, and we calculated a weighted averaged connectivity map as our final map. Habitat was highly permeable to movements between core areas of two genetically distinct subpopulations located in southeastern Ohio. We also identified potential dispersal corridors from the core areas to other regions of Ohio dominated by agriculture and suburban development via forested riparian corridors. Overall, our analysis offers new information on habitat selection and connectivity in a rebounding felid population and offers important ecological information for wildlife management strategies. We recommend that the suitability and connectivity models should be periodically updated until the population reaches an equilibrium, and be integrated with data from neighboring states for a comprehensive assessment of a conservation success story.

Human impacts on the environment and wildlife in California’s past: Lessons from California archaeology

The long history of human-animal interactions in California prior to European contact is frequently not considered when setting ecological baselines and, by consequence, when planning conservation and management expectations and strategies for native species. This article reviews archaeological perspectives that explore the relationship between human niche construction, plant and wildlife populations, and human health in pre-European contact Central California, with an emphasis on the Central Valley and Delta, the surrounding foothills, and the San Francisco Bay Area. A summary of the archaeological record for Central California is provided, along with how niche construction and related evolutionary based models have been used in prehistoric California. Examples of the influences of human niche construction on flora, fauna, and human health from the archaeological and ethnographic record are then discussed. This information is tied to modern wildlife research and management practices that would serve contemporary fish and wildlife management given that human influences on species “natural” habitats and ecological baselines extends much further into the past than current ecological baselines and wildlife management strategies traditionally recognize.

The Impact of Information on Attitudes toward Sustainable Wildlife Utilization and Management: A Survey of the Chinese Public.

Simple SummaryThe widespread dissemination of information related to wildlife utilization in new online media and traditional media undoubtedly impacts societal conservation concepts and attitudes, thus triggering public discussions on the relationship between conservation and utilization. In this study, questionnaires were distributed in seven major geographic regions of Chinese mainland to investigate the public’s awareness and agreement with information related to the utilization of wildlife in order to measure the impact of information on various issues relating wildlife conservation and utilization. The Chinese public had the greatest awareness and agreement with information that prevents unsustainable and illegal utilization, and the least awareness and agreement with information that promotes unsustainable utilization. It is also noteworthy that the Chinese public have higher levels of awareness and agreement with information that does not support utilization than with information that supports sustainable utilization. From our research, we can conclude that overall the public tends to support and be informed by a purist view of conservation, and there is significantly less support for conservation based on sustainable utilization. On this basis, we suggest that in the future, conservation education should seek to balance the public’s respect and love for nature which is often inspired by social media, with more scientific information about scientific understandings that influence conservation policy and practice.The widespread dissemination of information related to wildlife utilization in new online media and traditional media undoubtedly impacts societal conservation concepts and attitudes, thus triggering public discussions on the relationship between conservation and utilization. A study on how public attitudes and concepts are affected by the related information on wildlife utilization is helpful to implement the scientific wildlife conservation and management strategies, and to propose targeted measures to optimize the information environment. We designed the questionnaire to investigate the public’s awareness and agreement with related information on wildlife utilization so as to measure how information with different dissemination channels, source types, and content orientation influenced the public’s concept of wildlife conservation and utilization. The questionnaire was distributed in seven major geographical regions throughout China. Out of a total of 1645 questionnaires that were collected, 1294 questionnaires were valid, with an effective rate of 78.7%. Results show that respondents had the greatest awareness of information on preventing unsustainable and illegal utilization, and the lowest awareness of information on promoting unsustainable utilization, and that awareness of information that against utilization was higher than that of information which supported sustainable utilization. At the same time, respondents showed the greatest agreement for information on preventing unsustainable utilization and the lowest agreement for information on promoting unsustainable utilization; also, their agreement with information that against utilization was higher than that for information which supported sustainable use. Respondents had a high level of awareness of information on wildlife related to COVID-19 provided by experts. Gender, age, the level of development of the city in which they live, education, vegetarianism, and religious beliefs all affected respondents’ agreement with related information on wildlife utilization. This research suggests that the publicity and education of scientific conservation methods should be emphasized in the future conservation education. In addition, scholars in the field of wildlife research should assume the role of ‘influencer’ and give full play to the scientific guidance of public opinion.

Genetic characterization and implications for conservation of the last autochthonous Mouflon population in Europe

Population genetic studies provide accurate information on population structure, connectivity, and hybridization. These are key elements to identify units for conservation and define wildlife management strategies aimed to maintain and restore biodiversity. The Mediterranean island of Sardinia hosts one of the last autochthonous mouflon populations, descending from the wild Neolithic ancestor. The first mouflon arrived in Sardinia ~ 7000 years ago and thrived across the island until the twentieth century, when anthropogenic factors led to population fragmentation. We analysed the three main allopatric Sardinian mouflon sub-populations, namely: the native sub-populations of Montes Forest and Mount Tonneri, and the reintroduced sub-population of Mount Lerno. We investigated the spatial genetic structure of the Sardinian mouflon based on the parallel analysis of 14 highly polymorphic microsatellite loci and mitochondrial D-loop sequences. The Montes Forest sub-population was found to harbour the ancestral haplotype in the phylogeny of European mouflon. We detected high levels of relatedness in all the sub-populations and a mitochondrial signature of hybridization between the Mount Lerno sub-population and domestic sheep. Our findings provide useful insights to protect such an invaluable genetic heritage from the risk of genetic depletion by promoting controlled inter-population exchange and drawing informed repopulation plans sourcing from genetically pure mouflon stocks.

Stakeholders engagement as an important step for the long‐term monitoring of wild ungulate populations

Abstract Monitoring trends in animal populations is essential for the development of appropriate wildlife management strategies. However, long‐term studies are difficult to maintain mainly due to the lack of continuous funding. In this scenario, the collaboration between local stakeholders and researchers can be a fruitful partnership to monitor game species for long periods and vast territories. We present an experimental framework with the involvement of researchers, local hunters and game managers for the continuous monitoring of wild ungulate populations. By combining vehicle‐based counts with distance sampling techniques, we implemented and validated a sampling scheme able to provide demographic information for the effective management of wild ungulate populations. Here, we used an Iberian red deer (Cervus elaphus) population as a model. The project implementation involved 30 participants including 24 stakeholders and 6 field technicians/data analysts with experience in monitoring wild ungulates. A total of eight teams covered 29 itineraries, synchronously, in two periods of ecological relevance for red deer, early summer and early autumn. Density estimates were consistent among sampling periods and characterized by acceptable coefficients of variation (approximately 20%). Our results prove that the application of the proposed framework is feasible (three to four itineraries per team), cost‐ and time‐effective (one week per sampling period) and produce population estimates fit for management. Being based on direct observations, the method would provide important demographic indicators (e.g. population density, age structure and fawn recruitment, and group size) about wild ungulate populations. Apart from engaging interested stakeholders, the success of our proposal relies on three key actions including the theoretical and field instruction of participants, the definition of timely and unbiased survey designs and the maintenance of participants’ motivation. The implementation of rigorous and standardized sampling protocols is pivotal for data integration through time and space. In the absence of continuous funding, the voluntary collaboration between entities should be fostered to study and mitigate the potential threats to wild ungulate populations resulting from disease, unregulated hunting and environmental changes.

Wildlife roadkill in Odisha, India: the threat to biodiversity needs to be addressed

Over a 90–day period we assessed wildlife roadkill on National highway NH–16 in the state of Odisha, India. We recorded 123 vertebrate carcasses: 14 species of mammals, nine species of birds, and seven species of reptiles. Our findings reiterated NH–16 as a vulnerable site of roadkill of many native wild animals and birds. Further studies are required where linear infrastructure affects wildlife and appropriate management strategies are needed.